Thermo Fisher Scientific PLS3 Monoclonal Antibody (GT236)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT236

Immunogen

Recombinant protein encompassing a sequence within the center region of human T-Plastin. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735321

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2

Predicted Reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (99%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Phospholipid scramblases (PLS) translocate phospholipids bidirectionally between inner and outer leaflets of the plasma membrane and are implicated in apoptosis (1,2). Members of this family, PLS1 and PLS3 are both substrates of Protein kinase C (PKC) delta and are phosphorylated by PKC-delta during apoptosis. PKC-delta translocates to mitochondria during apoptosis and phosphorylates PLS3. Overexpression of PLS3 in the HEK293 cells enhanced apoptosis induced by UV-irradiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.