Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (LDK18), Alexa Fluor 488, eBioscience
인간 Cytokeratin 18을 인식하는 LDK18 단클론 항체로, Alexa Fluor 488 형광 표지. IHC, ICC/IF, Flow Cytometry 등 다양한 분석에 사용 가능. 488 nm/519 nm 형광 특성, 4°C 암소 보관. 종양 표지 및 세포형 구분 연구용 적합.
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Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (LDK18), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LDK18 |
| Conjugate | Alexa Fluor™ 488 |
| Additional Formats | Unconjugated, eFluor 570, Request custom conjugation |
| Excitation/Emission Max | 499/520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574480 |
Product Specific Information
The monoclonal antibody LDK18 recognizes human Cytokeratin 18, a member of the intermediate filament family found in epithelial cells. Cytokeratin 18 (type I) commonly pairs with Cytokeratin 8 (type II) to form keratin filaments. It serves as a tumor marker for identifying epithelial-origin tumors and metastases, and is expressed in proliferative retinal pigment epithelium cells.
Applications Reported:
Used in microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested:
Validated in MeOH-fixed human cells for ICC at ≤5 µg/mL. Optimal titration recommended per assay.
Fluorescence Characteristics:
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and pairs with Cytokeratin 8. It is expressed in epithelial tissues of the gastrointestinal, respiratory, and urogenital tracts, as well as endocrine and exocrine tissues. Found mainly in non-squamous epithelia and adenocarcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the KRT18 gene are associated with cryptogenic cirrhosis. Cytokeratin subtype patterns aid in tumor classification and differential diagnosis via immunohistochemistry, cytopathology, and flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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