Thermo Fisher Scientific CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (101)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody has specificity for Human RANKL/OPGL/TNFSF11/CD254.

Target Information

The CD254 (RANK Ligand) gene encodes a member of the tumor necrosis factor (TNF) cytokine family, which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It also acts as a dendritic cell survival factor and participates in T cell-dependent immune regulation.

T cell activation induces expression of this gene, leading to increased osteoclastogenesis and bone loss. This protein activates antiapoptotic kinase AKT/PKB via a signaling complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.

Targeted disruption of the related gene in mice results in severe osteopetrosis and absence of osteoclasts. Deficient mice exhibit defects in early T and B lymphocyte differentiation and fail to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been identified.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.