Thermo Fisher Scientific CD254 (RANK Ligand) Recombinant Rabbit Monoclonal Antibody (218)

Applications

• Western Blot (WB): 1:500–1:2,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened for optimal performance.

Advantages of recombinant rabbit monoclonal antibodies:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin–free formulation
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody specifically recognizes Human RANKL/OPGL/TNFSF11/CD254.

Target Information

The RANKL (TNFSF11/CD254) gene encodes a member of the TNF cytokine family. It acts as a ligand for osteoprotegerin and plays a key role in osteoclast differentiation and activation. It also functions as a dendritic cell survival factor and regulates T cell–dependent immune responses.

Activation of T cells induces expression of this gene, promoting osteoclastogenesis and bone loss. RANKL activates antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and TRAF6, suggesting a role in apoptosis regulation.

Disruption of the related gene in mice leads to severe osteopetrosis, absence of osteoclasts, defects in T and B lymphocyte differentiation, and failure to form mammary structures during pregnancy. Two alternatively spliced transcript variants have been identified.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.