Thermo Fisher Scientific Ki-67 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 66 publications 66 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.1 - 5 µg/mL

View 7 publications 7 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

0.5-1 µg/ml

View 14 publications 14 publications

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rabbit

Published species

Bovine, Dog, Horse, Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ki-67,uniProtId:P46013-1,ncbiNodeId:9606,antigenRange:1200-1300,antigenLength:3256,antigenImageFileName:PA5-19462_Ki-67_P46013-1_Rabbit.svg,antigenImageFileNamePDP:PA5-19462_Ki-67_P46013-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981523

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to tissue staining.

Recommended positive controls:

ICC/IF - Wildtype HAP1 cells, SK-N-SH cells, HeLa cells, MEF1 cells

IHC (P) - Mouse and human spleen tissue, human skin carcinoma tissue, human colon tissue, mouse tumor tissue, human skin tissue

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.