Thermo Fisher Scientific BCL2 Monoclonal Antibody (OTI10C7), TrueMAB

Applications

Western Blot (WB)

• Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

• Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

BCL-2 is a key regulator of apoptosis, functioning to inhibit or promote cell death. Members of the BCL-2 family can dimerize to modulate apoptosis.

• Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect.
• Bax and Bak are essential for cytochrome c release from mitochondria, initiating apoptosis.
• Bax promotes apoptosis, while BCL-2 suppresses it.

Constitutive expression of BCL2, such as through translocation to the Ig heavy chain locus, is associated with follicular lymphoma. In these lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, unlike normal or hyperplastic centers. Two transcript variants of BCL-2 differ in their C-terminal ends. Overexpression of BCL-2 is linked to cancers including B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.