Thermo Fisher Scientific HNF4A Monoclonal Antibody (OTI2H2), TrueMAB
HNF4A 단백질을 인식하는 Mouse Monoclonal 항체로, Western Blot 및 IHC(Paraffin) 실험에 적합합니다. Human 시료에 반응하며, Lyophilized 형태로 제공되어 장기 보관이 용이합니다. 재구성 후 약 1 mg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–315 of human HNF4A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4A) is an orphan nuclear receptor expressed in the liver, kidney, intestine, and pancreas.
Mutations in HNF4A are associated with maturity-onset diabetes of the young type 1 (MODY1).
HNF4 binds DNA as a homodimer and can produce up to nine isoforms (HNF4A1–HNF4A9) through alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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