Thermo Fisher Scientific VSIG4 Monoclonal Antibody (JAV4), PE-Cyanine7, eBioscience

Thermo Fisher Scientific VSIG4 Monoclonal Antibody (JAV4), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.06 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

This JAV4 monoclonal antibody recognizes human VSIG4 (V-Set and Immunoglobulin domain containing 4), also known as Complement Receptor of the Immunoglobulin superfamily (CRIg) or Z39Ig.
VSIG4 is a type I transmembrane glycoprotein related to the B7 family of immune regulatory proteins, expressed on tissue-resident and tumor-infiltrating macrophages. It binds complement components C3b and iC3b to inhibit the alternative complement pathway and support phagocytosis.
VSIG4 also suppresses T cell activation and IL-2 production, contributing to peripheral tolerance and inflammation control. Expression on tumor-infiltrating macrophages suggests a role in immune evasion.

• Recognizes both isoforms of human VSIG4
• Does not cross-react with mouse or rat VSIG4
• Tested for flow cytometric analysis of human monocyte-derived macrophages
• Use at 5 µL (0.06 µg) per test (100 µL final volume)
• Light-sensitive tandem dye — protect from light
• Compatible with fixation using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54)
• Excitation: 488–561 nm; Emission: 775 nm; Laser: Blue, Green, Yellow-Green
• 0.2 µm post-manufacturing filtered

Target Information

VSIG4 is a negative regulator of murine and human T cell proliferation and IL-2 production.
It is a member of the B7 family involved in modulation of T cell activation by antigen-presenting cells (APCs).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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