Thermo Fisher Scientific MERTK Monoclonal Antibody (DS5MMER), PE-Cyanine7, eBioscience

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: Not specified
• View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test
• View 15 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170–210 kDa member of the TAM family of tyrosine kinase receptors (Axl, Tyro3). MerTK is expressed on tissue macrophages and mediates removal of apoptotic cells through ligands Protein S and Gas6. Ligand binding induces autophosphorylation, activating PI3K and Akt pathways, leading to phagocytosis and inhibition of pro-inflammatory cytokine production.
MerTK deficiency causes autoimmunity and inflammation. It may serve as an entry receptor for filoviruses and helps discriminate macrophages from dendritic cells. MerTK is also expressed on malignant cells, contributing to immune evasion.

• Works on paraformaldehyde-fixed cells
• Reported Application: Flow Cytometry
• Tested Application: Flow Cytometry of mouse resident peritoneal cells (≤0.5 µg/test)
• Recommended titration for optimal performance
• Light sensitivity: Protect from light
• Fixation: Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to 3 days at 4°C in dark
• Excitation: 488–561 nm; Emission: 775 nm; Laser: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

MERTK (cMER) is a tyrosine kinase proto-oncogene involved in retinal pigment epithelium (RPE) phagocytosis and various physiological processes such as cell survival, migration, and efferocytosis. Ligand binding induces autophosphorylation and interaction with GRB2 or PLCG2, leading to activation of MAPK1/2, FAK/PTK2, and RAC1 pathways.
MERTK signaling regulates macrophage clearance of apoptotic cells, platelet aggregation, and cytoskeletal reorganization. In RPE, it mediates rod outer segment phagocytosis and inhibits TLR-mediated innate immune responses via STAT1 activation and SOCS1/3 induction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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