Takara Helper virus-free preparation of AAV particles to deliver Cas9 and sgRNA
상품 옵션 정보 | ||||||
---|---|---|---|---|---|---|
카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
632608 | Takara 632608 AAVpro® CRISPR/Cas9 Helper Free System (AAV2), 1 System pk | 재고문의 | pk | 1,707,000원 | 1,877,700원 | |
632619 | Takara 632619 AAVpro® CRISPR/SaCas9 Helper Free System (AAV2), 1 System pk | 재고문의 | pk | 1,674,000원 | 1,841,400원 | |
632609 | Takara 632609 AAVpro® CRISPR/Cas9 Vector System, 1 System pk | 재고문의 | pk | 822,000원 | 904,200원 | |
632618 | Takara 632618 AAVpro® CRISPR/SaCas9 Vector System, 1 System pk | 재고문의 | pk | 789,000원 | 867,900원 |
다른 상품 둘러보기
Overview
Viral delivery of the Cas9 gene and an sgRNA allows for genome editing in hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
AAV delivery of Cas9 precludes genomic integration and persistent expression of Cas9, reducing off-target effects
Expression of human microRNA miR-342 from the packaging plasmid significantly increases AAV titer
AAV Extraction Solution is also included for efficient AAV2 viral particle isolation
More Information
AAVpro CRISPR/Cas9 Helper Free Systems (with S. pyogenes Cas9)
The SpCas9 gene is split between pAAV-Guide-it-Up and pAAV-Guide-it-Down plasmids with a 1.6-kb region of homology; the homologous region results in recombination in target cells, producing a full-length SpCas9 gene
SpCas9 uses the Protospacer Adjacent Motif (PAM) sequence NGG where N is any base—see the AAVpro CRISPR/Cas9 Systems User Manual for details
The pAAV-Guide-it-Down plasmid is provided prelinearized, facilitating single-step cloning of sgRNAs with reduced background due to plasmid re-ligation
AAVpro CRISPR/SaCas9 Helper Free Systems (with S. aureus Cas9)
The SaCas9 gene fits onto a single plasmid, pAAV-Guide-it-1, which that also contains the sgRNA expression cassette
SaCas9 (from Staphylococcus aureus) uses the PAM sequence NNGRR(T) where N is any base, R is an A or G base, and the (T) is strongly preferred—see the AAVpro CRISPR SaCas9 Systems User Manual for details
The pAAV-Guide-it-1 plasmid is provided prelinearized, facilitating single-step cloning of sgRNAs with reduced background due to plasmid re-ligation
Applications
- AAV-based delivery of a user-defined sgRNA and Cas9 for mammalian genome editing using CRISPR/Cas9 technology
Components
- AAVpro CRISPR/Cas9 Helper Free System (AAV2) (Cat. # 632608)
- AAVpro CRISPR/Cas9 Vector Set
- Guide-it Ligation Components
- Stellar Competent Cells
- pRC2-mi342 Vector
- pHelper Vector
- AAVpro Extraction Solution
- AAVpro CRISPR/Cas9 Vector System (Cat. # 632609)
- AAVpro CRISPR/Cas9 Vector Set
- Guide-it Ligation Components
- Stellar Competent Cells
- AAVpro CRISPR/SaCas9 Helper Free System (AAV2) (Cat. # 632619)
- AAVpro CRISPR/SaCas9 Vector Set
- Guide-it Ligation Components
- Stellar Competent Cells
- pRC2-mi342 Vector
- pHelper Vector
- AAVpro Extraction Solution
- AAVpro CRISPR/SaCas9 Vector System (Cat. # 632618)
- AAVpro CRISPR/SaCas9 Vector Set
- Guide-it Ligation Components
- Stellar Competent Cells
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
배송/결제/교환/반품 안내
배송 정보
기본 배송비 |
| 교환/반품 배송비 |
|
---|---|---|---|
착불 배송비 |
| ||
교환/반품 배송비 |
|
결제 및 환불 안내
결제 방법 |
|
---|---|
취소 |
|
반품 |
|
환급 |
|
교환 및 반품 접수
교환 및 반품 접수 기한 |
|
---|---|
교환 및 반품 접수가 가능한 경우 |
|
교환 및 반품 접수가 불가능한 경우 |
|
교환 및 반품 신청
교환 절차 |
|
---|---|
반품 절차 |
|