Thermo Fisher Scientific GADD45B Polyclonal Antibody, FITC
FITC로 결합된 GADD45B 폴리클로날 항체로 인간, 생쥐, 원숭이, 랫트 반응성. Western blot, IHC, ELISA, IP에 적합. 합성 펩타이드 면역원 사용, 형광 분석용. -20°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific GADD45B Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–160 on mouse GADD45b protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, allowing time for repair before S and M phases. The p53 tumor suppressor regulates the G1 checkpoint and upregulates GADD45 and p21 expression.
GADD45 binds Cdks and PCNA, stimulating DNA excision repair and inhibiting S phase entry.
GADD45-like proteins (GADD45β, GADD45γ) are induced by environmental stresses and activate p38/JNK via MEKK4.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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