Thermo Fisher Scientific GADD45B Polyclonal Antibody, Biotin
GADD45B 단백질을 인식하는 Biotin 결합 토끼 Polyclonal 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. 인간, 마우스, 랫드, 영장류 반응성. 합성 펩타이드 면역원 사용, 친화 크로마토그래피 정제. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–160 on mouse GADD45b protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, allowing time for DNA repair before entry into S and M phases. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD45 and p21.
GADD45 binds both Cdks and PCNA, proteins involved in DNA replication and repair. It stimulates DNA excision repair in vitro and inhibits entry of cells into S phase, suggesting GADD45 serves as a link between the p53-dependent cell cycle checkpoint and DNA repair.
GADD45-like proteins, GADD45 beta and GADD45 gamma, are induced by environmental stresses and are thought to activate p38/JNK pathways via MEKK4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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