Thermo Fisher Scientific Ninein Monoclonal Antibody (79.160-7)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

79.160-7

Immunogen

Synthetic peptide derived from an internal region of the human ninein protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533504

Target Information

Ninein is a component of subdistal appendages of the mother centriole, localized specifically in the pericentriolar matrix of the centrosome. It may be involved in microtubule minus-end capping, centriole positioning, and protein anchoring. Recent studies have shown that the microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. At least five human ninein isoforms with divergent C-terminal domains and two mouse isoforms have been reported. Data indicates that the combined action of domains (C-terminal and N-terminal) might, in the absence of coiled coil region, be sufficient to localize ninein to the mother centriole. Microinjection of antibodies against ninein into metaphase HeLa cells can disrupt the reformation of tubular conformation of proteins within the centrosome following cell division, and consequently lead to dispersal of centrosomal material throughout the cytosol. MTOC (microtubule organizing center) function can be disrupted when anti-ninein antibodies are injected into postmitotic cells. Antibodies against ninein are present in sera from patients with autoimmune diseases that develop autoantibodies to centrosomal proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.