Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (mN1A)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

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Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

-

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Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

mN1A

Immunogen

Recombinant protein derived from the intracellular domain of mouse Notch1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533505

Product Specific Information

This antibody is specific for the Notch1 (neurogenic locus notch homolog protein1, Motch A, mT14, p300) protein. This antibody gives a band of ~65 kDa on Western blot analysis using Notch1-IC OP transfected cells. Western blot analysis using Jurkat cells expresses the target band at ~110-120 kDa, with an additional band occasionally observed at ~220kDa. This antibody does not cross-react with Notch 2,3, and 4 as assessed by Western blot analysis.

Target Information

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.