Thermo Fisher Scientific GRB2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.05-0.15 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the C terminus amino acids PRNYVTPVNRNV (aa 206-217). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GRB2,uniProtId:P62993-1,ncbiNodeId:9606,antigenRange:206-217,antigenLength:217,antigenImageFileName:PA1-9051_GRB2_P62993-1_Goat.svg,antigenImageFileNamePDP:PA1-9051_GRB2_P62993-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2263580

Product Specific Information

This antibody is expected to recognize both reported isoforms (NP_002077.1 and NP_987102.1).

This antibody is predicted to react with bovine and canine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Target Information

GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.