Thermo Fisher Scientific GRB2 Polyclonal Antibody
GRB2 단백질을 인식하는 Thermo Fisher Scientific의 Polyclonal 항체로, Western Blot 및 IHC(Paraffin)에서 검증됨. Human, Mouse, Pig, Rat에 반응하며, C-말단 합성 펩타이드 면역원 사용. 액상 형태로 0.5 mg/mL 농도, -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.05–0.15 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C terminus amino acids PRNYVTPVNRNV (aa 206–217) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2263580 |
Product Specific Information
- This antibody is expected to recognize both reported isoforms (NP_002077.1 and NP_987102.1).
- Predicted to react with bovine and canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
GRB2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB2–Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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