Thermo Fisher Scientific HPK1 Polyclonal Antibody
HPK1 단백질을 인식하는 Thermo Fisher Scientific의 산양 유래 폴리클로날 항체. Western blot 및 IHC(P) 분석에 적합하며, 인간에 반응하고 다른 종에서도 예측 반응. 액상 형태, 0.5 mg/mL 농도, -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 0.5–2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N terminus amino acids DVVDPDIFNRDPR (aa 2–15) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_1076649 |
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
Tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
MAP4K1 (also known as HPK1, Hematopoietic progenitor kinase 1) is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase superfamily.
It acts as a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway and diminishes T cell receptor (TCR) signaling and proliferation by phosphorylating the adaptor protein SLP-76.
HPK1 activates JNK1 specifically and increases AP-1-mediated transcriptional activity.
Unlike PAK65, HPK1 does not bind Rac1 or Cdc42, indicating a distinct activation mechanism within hematopoietic signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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