Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 Goat Polyclonal 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간 및 비인간 영장류 반응성이 있으며, 단백질 로딩 컨트롤용으로 널리 사용됩니다. 0.5 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–6 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminus residues H Q V V S S D F N S D T (aa 279–290) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1074703 |
Product Specific Information
- This antibody is predicted to react with canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in diverse cellular processes such as DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling. It is also implicated in apoptosis, neurodegeneration, and cancer-related pathways.
Due to its high expression across tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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