Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat VEGF 165. Anti-Rat VEGF 165-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat VEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat VEGF (500-P275) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat VEGF.

Western Blot:
To detect Rat VEGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Rat VEGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P275BT-1MG will be provided as 2 × 500 µg.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are fundamental regulators in the cell signaling of angiogenesis. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine linked to this process.
Five different isoforms of VEGF have been described, generated by alternative splicing from a single VEGF gene. These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which are expressed almost exclusively on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.