Thermo Fisher Scientific PTHLH Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930200

Product Specific Information

AA Sequence of recombinant protein: AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PTHrP. Anti-Human PTHrP-specific antibody was purified by affinity chromatography employing an immobilized Human PTHrP matrix.

Sandwich ELISA: To detect Human PTHrP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PTHrP (500-P276Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PTHrP.

Western Blot: To detect Human PTHrP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PTHrP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The protein encoded by this gene is a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, in-frame and downstream of the initiator AUG codon, to give rise to nuclear forms of this hormone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.