Thermo Fisher Scientific PTHLH Polyclonal Antibody, PeproTech
Human PTHrP 단백질을 검출하기 위한 Rabbit Polyclonal Antibody. Western Blot과 ELISA에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며, 높은 특이성과 민감도를 보유.
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Thermo Fisher Scientific PTHLH Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human PTHrP |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930200 |
Product Specific Information
AA Sequence of recombinant protein:
AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PTHrP. Anti-Human PTHrP-specific antibody was purified by affinity chromatography employing an immobilized Human PTHrP matrix.
Sandwich ELISA:
To detect Human PTHrP by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PTHrP (500-P276Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human PTHrP.
Western Blot:
To detect Human PTHrP by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human PTHrP is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The protein encoded by this gene is a member of the parathyroid hormone family. It regulates endochondral bone development and epithelial-mesenchymal interactions during formation of mammary glands and teeth. This hormone is involved in lactation, possibly by regulating calcium mobilization and transfer to milk. The receptor, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG start sites, resulting in nuclear forms of this hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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