Thermo Fisher Scientific EGF Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific EGF Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilutions

• Western Blot (WB): 0.5–1.0 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein:
  MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR
• Preparation:
  Produced from sera of rabbits immunized with highly pure Recombinant Rat EGF. Anti-Rat EGF-specific antibody was purified by affinity chromatography and then biotinylated.
• Sandwich ELISA:
  To detect Rat EGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat EGF (500-P277) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat EGF.
• Western Blot:
  To detect Rat EGF by Western Blot, use at 0.5–1.0 µg/mL. Detection limit for Recombinant Rat EGF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
• Packaging:
  500-P277BT-1MG will be provided as 2 × 500 µg.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF Receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR initiates multiple signaling pathways in response to environmental stimuli or ligand binding. EGFR can form homo- or heterodimers, each triggering distinct signaling cascades via SH2-domain-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. It activates several downstream pathways such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT modules.
EGF may also influence NF-κB signaling. Defects in the EGF gene are linked to hypomagnesemia type 4, and dysregulation is associated with certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.