Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats:

• Unconjugated
• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation available

Product Specific Information

The TS2/16 monoclonal antibody reacts with human CD29 (integrin beta 1), a 130 kDa transmembrane glycoprotein. CD29 forms heterodimers with integrin alpha subunits to create VLA adhesion molecules involved in cell-cell and cell-matrix interactions. It is expressed broadly on lymphocytes and monocytes, with lower levels on granulocytes. TS2/16 exhibits activating activity for beta 1 integrins.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells; use 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample to antibody cocktails before combining with cells.

NovaFluor Technology:
Based on Phiton™ dye structures engineered for minimal spillover and optimized fluorescence signatures.
Excitation: 639 nm; Emission: 710 nm; Laser: 633–640 nm (Red).

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29)
A 110 kDa surface glycoprotein expressed on various cell types including leukocytes. Forms heterodimers with multiple alpha chains (α1–α6) to define VLA integrins. Plays a role in:

• Cell adhesion and migration
• Immune cell trafficking
• Tumor cell drug resistance
• Inflammatory response regulation

Two major isoforms exist: β1A (ubiquitous except cardiac/skeletal muscle) and β1D (muscle-specific).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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