Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The TS2/16 monoclonal antibody reacts with human CD29 (integrin beta 1), a ~130 kDa single-pass transmembrane glycoprotein. CD29 forms heterodimers with integrin alpha subunits to create the VLA (very late antigen) family of adhesion molecules, mediating cell-cell and cell-matrix adhesion. It is broadly expressed on lymphocytes and monocytes, and at lower levels on granulocytes. The TS2/16 antibody exhibits activating activity for beta 1 integrins.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Recommended usage is 5 µL (0.5 µg) per 100 µL test volume. Cell count range: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• This product ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which enhances staining performance.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample prior to cell staining. For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, employing engineered nucleic acid dye structures that minimize spectral spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red laser)

Target Information

Gene: ITGB1 (Integrin Subunit Beta 1, CD29)
Protein: 110 kDa cell surface glycoprotein
Expression: Widely expressed in leukocytes, platelets, and other cell types
Function: Forms heterodimers with alpha chains (α1–α6) to create VLA integrins, mediating adhesion and migration processes.
Clinical Relevance:

• Decreased ITGB1 expression correlates with tumor multidrug resistance.
• Upregulated during leukocyte migration and inflammation.
• Involved in tissue damage regulation and inflammatory bowel disease.
• Modulates cytokine secretion in airway smooth muscle via fibronectin and collagen interaction.
• Isoforms:* Two major forms, β1A (ubiquitous) and β1D (muscle-specific).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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