Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), NovaFluor Blue 510, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation available

Product Specific Information

The TS2/16 monoclonal antibody reacts with human CD29 (integrin beta 1), an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 forms heterodimers with integrin alpha subunits to create the VLA (very late antigen) subfamily of adhesion molecules, involved in cell-cell and cell-matrix adhesion. CD29 is broadly expressed on lymphocytes and monocytes, with lower expression on granulocytes. The TS2/16 antibody has activating activity for beta 1 integrins.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.

NovaFluor Technology:
Based on Phiton™ technology using nucleic acid dye structures for engineered fluorescent signatures with minimized spillover and spread.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

Gene: ITGB1 (Integrin Subunit Beta 1, CD29)
Protein: 110 kDa cell surface glycoprotein
Expression: Widely expressed in leukocytes, platelets, and various cell types
Function: Forms heterodimers with alpha chains (α1–α6) to determine binding properties of VLA integrins; mediates adhesion to extracellular matrix proteins.
Clinical relevance:

• Decreased ITGB1 expression linked to tumor multidrug resistance
• Upregulated during leukocyte migration and inflammation
• Plays roles in immune cell trafficking, tissue damage regulation, and inflammatory bowel disease
• Enhances cytokine secretion in airway smooth muscle via fibronectin and collagen interactions
• Two major isoforms: β1A (ubiquitous) and β1D (muscle-specific)

For Research Use Only. Not for use in diagnostic procedures.