Thermo Fisher Scientific Rat anti-Mouse IgG2a Secondary Antibody, Super Bright 600, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 1.0 µg/test

Product Specifications

Additional Formats

• APC
• Biotin
• eFluor 570
• FITC
• HRP
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody m2a-15F8 recognizes mouse IgG2a antibodies and serves as a secondary reagent in flow cytometry and microscopy. It does not cross-react with other mouse isotypes or rat immunoglobulins.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on normal human peripheral blood cells; ≤1.0 µg/test (100 µL final volume). Optimal titration recommended.

Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended detection filter: 610/20 bandpass. Ensure instrument compatibility.

When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark with minimal signal loss.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Anti-Mouse secondary antibodies are affinity-purified with well-defined specificity for mouse immunoglobulins. They are valuable for detection, sorting, or purification of target molecules. Secondary antibodies enhance sensitivity and versatility by enabling signal amplification and compatibility with multiple detection systems (e.g., HRP, AP, fluorescence).

Produced by immunizing host animals with pooled mouse immunoglobulins and further purified via immunoaffinity chromatography or conjugation for high specificity.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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