Thermo Fisher Scientific Fbp5A Polyclonal Antibody
Thermo Fisher Scientific의 Fbp5A 폴리클로날 항체는 인간 Fbp5A 단백질을 인식하며 Western blot, IHC, ELISA, IP 등에 사용 가능합니다. 친화 크로마토그래피로 정제된 고순도 항체로, -20°C에서 안정적으로 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500 – 1:3,000 |
| ELISA | 1:5,000 – 1:20,000 |
| Immunoprecipitation (IP) | 1:500 – 1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to a region near the amino terminal end of human Fbp5A protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.3 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human Fbp5A protein. A BLAST analysis suggests cross-reactivity with Fbp5A protein from human and chimpanzee based on 100% homology with the immunizing sequence. Partial reactivity is expected with Fbp5A from dog (~92% sequence homology). Reactivity against homologues from other species is not known.
Target Information
F-box proteins are critical components of the conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), which recruits substrates for ubiquitination and degradation through specific protein-protein interaction domains. Novel human F-box proteins such as FBG1, FBG2, FBG3, FBG4, and FBG5 form a subfamily of mammalian F-box proteins. These FBG genes are expressed in limited human tissues, including kidney, liver, brain, and muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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