Thermo Fisher Scientific APG7 Polyclonal Antibody
Human Apg7 단백질을 인식하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. Affinity chromatography로 정제된 액상 항체로, -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:300,000 – 1:400,000 |
| Immunoprecipitation (IP) | 1:500 – 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 550–575 of Human Apg7 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human Apg7 protein. BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen (G-D-S-T-R-D-R-T) are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Target Information
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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