Thermo Fisher Scientific beta TrCP2 Polyclonal Antibody
Thermo Fisher Scientific의 beta TrCP2 Polyclonal Antibody는 인간 및 다양한 종의 betaTrCP2 단백질을 검출하는 토끼 다클론 항체입니다. Western blot, ICC/IF, ELISA, IP에 적합하며, 고순도의 친화 크로마토그래피 정제 항체로 연구용에 최적화되어 있습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:10,000 – 1:100,000 |
| Immunoprecipitation (IP) | 1:200 – 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human ßTrCP2 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with human betaTrCP2 protein. A BLAST analysis suggests cross-reactivity with betaTrCP2 from human, mouse, dog, bovine, chimpanzee, chicken, and horse based on 100% homology with the immunizing sequence. Cross-reactivity with betaTrCP2 from other sources has not been determined.
Target Information
This gene encodes a member of the F-box protein family, characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are subunits of the ubiquitin protein ligase complex SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. They are divided into three classes:
- Fbws: containing WD-40 domains
- Fbls: containing leucine-rich repeats
- Fbxs: containing other interaction modules or no recognizable motifs
The encoded protein belongs to the Fbws class, containing multiple WD40 repeats in addition to an F-box. This gene contains at least 14 exons, and alternative splicing generates 3 transcript variants differing by the presence or absence of two alternate exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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