Thermo Fisher Scientific UBE2J1 Monoclonal Antibody (OTI1F6), TrueMAB
UBE2J1 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, WB, IHC(P), Flow Cytometry에 적합합니다. Human 시료 반응성이 있으며, 동결건조 형태로 제공됩니다. 단백질 품질 관리 연구 및 유비퀴틴 관련 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F6 |
| Immunogen | Full length human recombinant protein of human UBE2J1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The modification of proteins with ubiquitin is a key cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves three classes of enzymes:
- Ubiquitin-activating enzymes (E1)
- Ubiquitin-conjugating enzymes (E2)
- Ubiquitin-protein ligases (E3)
UBE2J1 encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum and may contribute to ER-associated degradation via the ubiquitin-proteasome system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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