
Thermo Fisher Scientific UBE2J1 Monoclonal Antibody (OTI1H10), TrueMAB
UBE2J1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 최적화됨. HEK293T 세포에서 발현된 full-length 인간 단백질을 면역원으로 사용. 동결건조 형태로 제공되며, 1 mg/mL 농도로 재구성 가능. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H10 |
| Immunogen | Full length human recombinant protein of human UBE2J1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) before use.
Target Information
The modification of proteins with ubiquitin is a key cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves three classes of enzymes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
The UBE2J1 gene encodes a member of the E2 ubiquitin-conjugating enzyme family.
This enzyme is located in the membrane of the endoplasmic reticulum and may contribute to quality control and ER-associated degradation by the ubiquitin-proteasome system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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