Thermo Fisher Scientific POFUT2 Monoclonal Antibody (OTI3A12), TrueMAB
POFUT2 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot과 Flow cytometry에 최적화됨. HEK293T에서 생산된 full-length human recombinant protein을 면역원으로 사용. Lyophilized 형태, 1 mg/mL 농도, PBS buffer와 trehalose 포함. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Flow Cytometry (Flow)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A12 |
| Immunogen | Full length human recombinant protein of human POFUT2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Fucose is typically found as a terminal modification of branched chain glycoconjugates and also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor-like repeats or thrombospondin type-1 repeats.
POFUT2 is an O-fucosyltransferase that utilizes THBS type-1 repeats as substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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