Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), APC, eBioscience

Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 4 publications

Product Specifications

Available Formats

• Unconjugated
• Biotin
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eB-h209 monoclonal antibody reacts with human CD209 (DC-SIGN), a 44 kDa type II transmembrane protein containing a C-type lectin binding domain.
DC-SIGN binds ICAM-3, ICAM-2, and HIV virus, and is preferentially expressed on human dendritic cells. It is thought to function as a receptor for HIV capture and transmission to T cells, supporting the primary immune response.
The eB-h209 clone was developed against a C-terminal peptide of human DC-SIGN.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of cultured human dendritic and peripheral blood cells
Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD209 gene encodes a transmembrane receptor known as DC-SIGN, expressed on dendritic cells and macrophages.
This receptor is part of the innate immune system, recognizing diverse pathogens such as parasites and viruses.
The protein comprises:

• N-terminal transmembrane domain
• Tandem-repeat neck domain
• C-type lectin carbohydrate recognition domain

The extracellular region mediates both pathogen recognition and cell adhesion via carbohydrate ligand binding.
Neck domain variations affect ligand binding avidity.
Closely related to L-SIGN (GeneID 10332), differing in ligand-binding properties and tissue distribution.
Alternative splicing results in multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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