Thermo Fisher Scientific Phospho-p90 RSK1 (Ser732) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3000

-

ELISA (ELISA)

1:5,000-1:50,000

-

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RSK1,uniProtId:Q15418-1,ncbiNodeId:9606,antigenRange:735,antigenLength:735,antigenImageFileName:600-401-B30_RSK1_Q15418-1_Rabbit.svg,antigenImageFileNamePDP:600-401-B30_RSK1_Q15418-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on >90% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.

Target Information

RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.