Takara Embgenix ESM Screen Kit

The Embgenix ESM Screen Kit is a Research Use Only (RUO) kit designed to evaluate chromosome copy number variation (CNV) using noninvasive samples collected during an IVF cycle, including embryo spent media (ESM) and/or blastocoel fluid. All 24 chromosomes (22 autosomes + 2 sex chromosomes) can be screened for the identification of whole-chromosome and segmental aneuploidies, as well as mosaicism (with limited thresholds).

The Embgenix ESM Screen Kit is a Research Use Only (RUO) kit designed to evaluate chromosome copy number variations (CNVs) using noninvasive samples collected during an IVF cycle, including embryo spent media (ESM) and/or blastocoel fluid. All 24 chromosomes (22 autosomes + 2 sex chromosomes) can be screened for the identification of whole-chromosome and segmental aneuploidies, as well as mosaicism (with limited thresholds).

This kit was optimized for handling larger volumes of the input material, and it comes with a quantification kit for the accurate determination of cfDNA present in noninvasive samples. This kit uses next-generation sequencing (NGS) technology as the detection method and is compatible with all Illumina sequencers.

To streamline the generation of sequencing libraries, the kit utilizes the gold-standard PicoPLEX whole genome amplification (WGA) and patented library-prep technology with unique dual indexes (UDIs). PicoPLEX quasi-random priming technology is widely recognized for accurate and reproducible detection of aneuploidies and CNVs from single cells and low-input DNA. The simple protocol dramatically speeds time to results while reducing handling errors and background. The library-prep workflow (fragmentation, repair, ligation, amplification, and indexing) completes in about two hours in a single tube. No intermediate purification steps or sample transfers are necessary, minimizing the potential for loss and mix-up of samples.

After sequencing, data analysis and reporting are performed using our specialized, cloud-based bioinformatics pipeline, Embgenix Analysis Software (RUO), with its advanced ESM workflow designed for noninvasive samples. The algorithm accurately determines CNVs by using bin counts to calculate chromosome copy numbers (CCNs) against an internally configured reference. The software provides options for automated and/or manual calls for whole-chromosome and segmental aneuploidies, as well as mosaicism. Data can be conveniently visualized in a chart or an idiogram plot. The software supports downloadable PDF reports, which can be customized for individual laboratory needs.

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Overview

• Complete workflow for CNV analysis of noninvasive samples collected in an IVF lab, including spent embryo culture media and blastocoel fluid
• PicoPLEX WGA technology for successful amplification of low-input and fragmented DNA
• Single-step WGA with reduced hands-on time and library prep with enzymatic fragmentation
• Larger input volumes for increased resolution, accommodating 30 μl of culture medium
• Scalable workflow for multiple throughputs (24–96 samples)
• Compatible with MiSeq® and NextSeq® 500/550 Illumina sequencers
• Increased sequencing power with UDIs included to allow pooling of multiple samples and reliable sequencing
• Data processed easily using Embgenix Analysis Software (RUO) with the advanced ESM workflow option, which is optimized for noninvasive samples

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Applications

• CNV detection in embryo spent media and blastocoel fluid
• Preimplantation genetic testing for aneuploidies in research samples

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.