Takara Embgenix ESM Screen Kit

Takara Embgenix ESM Screen Kit

The Embgenix ESM Screen Kit is a Research Use Only (RUO) kit designed to evaluate chromosome copy number variations (CNVs) using noninvasive samples collected during an IVF cycle, including embryo spent media (ESM) and/or blastocoel fluid.
All 24 chromosomes (22 autosomes + 2 sex chromosomes) can be screened for the identification of whole-chromosome and segmental aneuploidies, as well as mosaicism (within limited thresholds).

Key Features

• Complete workflow for CNV analysis of noninvasive IVF samples (ESM and blastocoel fluid)
• PicoPLEX WGA technology for amplification of low-input and fragmented DNA
• Single-step WGA and enzymatic fragmentation for reduced hands-on time
• Supports larger input volumes (up to 30 μl of culture medium)
• Scalable workflow for 24–96 samples
• Compatible with Illumina MiSeq® and NextSeq® 500/550 sequencers
• UDIs included for multiplexing and reliable sequencing
• Data processed using Embgenix Analysis Software (RUO) optimized for noninvasive samples

Kit Description

This kit is optimized for handling larger volumes of input material and includes a quantification kit for accurate determination of cfDNA in noninvasive samples.
Utilizing next-generation sequencing (NGS) technology, it is compatible with all Illumina sequencers.

The kit employs PicoPLEX whole genome amplification (WGA) and patented library-prep technology with unique dual indexes (UDIs).
PicoPLEX quasi-random priming ensures accurate and reproducible detection of aneuploidies and CNVs from single cells and low-input DNA.
The simple protocol accelerates results, reduces handling errors, and minimizes background noise.

The library-prep workflow (fragmentation, repair, ligation, amplification, and indexing) completes in about two hours in a single tube, without intermediate purification or sample transfers—reducing the risk of sample loss or mix-up.

Data Analysis

After sequencing, data analysis and reporting are performed with Embgenix Analysis Software (RUO), featuring an advanced ESM workflow for noninvasive samples.
The algorithm accurately determines CNVs by calculating chromosome copy numbers (CCNs) against an internal reference.
It supports both automated and manual calls for aneuploidy and mosaicism detection.
Results are visualized in charts or idiogram plots, and customizable PDF reports can be generated for laboratory use.

Applications

• CNV detection in embryo spent media and blastocoel fluid
• Preimplantation genetic testing for aneuploidies in research samples

Additional Product Information

Refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
The Product Components List and Certificates of Analysis can be found under the Documents tab.