Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), PE-Cyanine7, eBioscience
인간 및 생쥐 c-Maf 단백질을 인식하는 단클론 항체로, PE-Cyanine7 형광 표지. Flow Cytometry에 최적화되어 있으며, 0.5 µg/test 권장. IL-4, IL-21 등 T세포 분화 관련 연구에 적합. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811795 |
Available Formats
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 480
- eFluor 450
- eFluor 660
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. It is crucial for IL-4 expression in Th2 cells and plays roles in Th17 and Tfh cell differentiation, regulating IL-21, IL-23R, and CXCR5 expression.
Applications Reported:
This antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Recommended ≤ 0.5 µg/test (in 100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Antibody titration recommended for optimal performance.
Light Sensitivity:
PE-Cyanine7 tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness.
Excitation/Emission:
488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green Lasers
Target Information
c-Maf is a basic leucine zipper transcription factor, cellular counterpart of oncogenic v-maf. It regulates IL-4 in Th2 cells, crystalline genes in lens cells, insulin gene in islets, and p53/L7 through Maf recognition elements (MARE). It plays a role in Th2 differentiation, hematopoiesis, and IL-10 expression in macrophages. Chromosomal aberrations involving maf are found in multiple myeloma, and expression is observed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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