Thermo Fisher Scientific PKR Recombinant Rabbit Monoclonal Antibody (23H52L96)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-3 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Hamster

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

23H52L96

Immunogen

A recombinant protein corresponding to amino acids 54-158. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKR,uniProtId:P19525-1,ncbiNodeId:9606,antigenRange:54-158,antigenLength:551,antigenImageFileName:700286_PKR_P19525-1_Rabbit.svg,antigenImageFileNamePDP:700286_PKR_P19525-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532313

Product Specific Information

This antibody is predicted to react with Rhesus monkey based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.