Thermo Fisher Scientific EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Product Specific Information

This antibody is predicted to react with canine, mouse, and rat based on sequence homology.
On a non-reducing gel, intact IgG appears as a ~150 kDa band, and upon reduction, generates ~25 kDa (light chain) and ~50 kDa (heavy chain) bands.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Enhanced specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression is associated with multiple cancer types, making EGFR a major therapeutic target.
Encoded by the EGFR gene on chromosome 7, EGFR belongs to the HER/ERbB family (ERbB2, ERbB3, ERbB4). Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation, activating downstream signaling pathways.
Overexpression is frequently observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.