Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Request custom conjugation

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 (also known as NTB-A) is expressed on NK, T, and B cells. Engagement of Ly108 enhances NK cell activity and IFN-γ expression, and is implicated in X-linked lymphoproliferative disease.
CD4+ T cells from Ly108 knockout mice show reduced IL-4 production and defective neutrophil functions.
Staining has shown no strain differences between C57BL/6 and BALB/c mice.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes and thymocytes. Recommended ≤ 0.8 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize by titration for best performance.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance.
Use 5 µL per sample (regardless of number of NovaFluors). For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology, employing novel nucleic acid dye structures for engineered fluorescent signatures optimized for reduced spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red Laser)

Target Information

Ly108 is the mouse homologue of human SLAMF6. Expressed on NK, B, and T cells, Ly108 activation enhances NK cell activity and IFN-γ expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지