Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 755, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.8 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Request custom conjugation available

Product Specific Information

Description:
The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 (also known as NTB-A) is expressed on NK, T, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and IFN-gamma expression, and contributes to the inability of NK cells to kill Epstein-Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knockout mice show reduced IL-4 production and defective neutrophil functions.

Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes and thymocytes. Recommended ≤ 0.8 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• NovaFluor conjugates now ship with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates use Phiton™ technology, based on novel nucleic acid dye structures engineered to minimize spectral spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

Ly108 is the mouse homologue of human SLAMF6, expressed on NK, B, and T cells. Activation of Ly108 enhances NK cell activity and IFN-gamma expression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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