Thermo Fisher Scientific Myelin Basic Protein Monoclonal Antibody (MBP/4275)
Myelin Basic Protein(MBP)을 인식하는 Rat monoclonal antibody로, Western blot, IHC, ICC, ELISA에 사용 가능. 다양한 종에 반응하며, Protein A/G로 정제된 1 mg/mL 액상 항체. PBS(pH 7.4) buffer, 방부제 무첨가, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL | |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Chicken, Guinea pig, Human, Mammal, Sheep, Pig, Rabbit, Rat |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MBP/4275 |
| Immunogen | Spleen cells from an immunised outbred rat were fused with cells of the mouse NS0 myeloma cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP-related transcripts are also present in the bone marrow and immune system. These mRNAs arise from the long MBP gene (also called Golli-MBP), which contains three additional exons upstream of the classic MBP exons.
Alternative splicing from the Golli and MBP transcription start sites gives rise to two sets of MBP-related transcripts and gene products:
- Golli mRNAs – contain three exons unique to Golli-MBP, spliced in-frame to one or more MBP exons, encoding hybrid proteins with N-terminal Golli and C-terminal MBP sequences.
- MBP mRNAs – contain only MBP exons and produce the well-characterized myelin basic proteins.
This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit, important for gene regulation and function.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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