Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 570, eBioscience
인간 및 개의 Ki-67 단백질을 인식하는 단클론 항체로, 세포 증식 마커 분석에 적합합니다. eFluor 570 형광 표지로 면역조직화학(IHC) 및 면역세포화학(ICC/IF)에 사용됩니다. 높은 특이성과 안정성으로 암세포 성장률 평가에 활용됩니다.
- 카탈로그번호
- 41-5699-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 570, eBioscience
Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 570, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 570 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20Raj1 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11220088 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 615 / 660
- FITC
- PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms (345 and 395 kDa) are expressed in dividing cells and are detectable during active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0).
During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.
Ki-67 expression serves as a marker for determining the fraction of proliferating cells in tumor studies.
This antibody also recognizes canine Ki-67.
Applications Reported:
- Immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections
- Immunocytochemistry
Applications Tested:
- Tested by immunocytochemistry on fixed and permeabilized HeLa cells
- Recommended ≤ 5 µg/mL, titrate for optimal performance
Filter Recommendation:
Use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor™ 555 or TRITC filters are acceptable.
Excitation: 555 nm; Emission: 570 nm
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases. It associates with euchromatin and heterochromatin regions and shows cell cycle-dependent localization.
Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis.
In neoplastic tissues, Ki-67 index correlates with tumor grade and is a valuable prognostic marker.
Commonly used in neuronal cell cycle and proliferation studies.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.