Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 570, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 570, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / 615 / 660
• FITC
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms (345 and 395 kDa) are expressed in dividing cells and are detectable during active phases of the cell cycle (G1, S, G2, and mitosis), but absent in resting cells (G0).
During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.
Ki-67 expression serves as a marker for determining the fraction of proliferating cells in tumor studies.

This antibody also recognizes canine Ki-67.

Applications Reported:

• Immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections
• Immunocytochemistry

Applications Tested:

• Tested by immunocytochemistry on fixed and permeabilized HeLa cells
• Recommended ≤ 5 µg/mL, titrate for optimal performance

Filter Recommendation:
Use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor™ 555 or TRITC filters are acceptable.
Excitation: 555 nm; Emission: 570 nm

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases. It associates with euchromatin and heterochromatin regions and shows cell cycle-dependent localization.
Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis.
In neoplastic tissues, Ki-67 index correlates with tumor grade and is a valuable prognostic marker.
Commonly used in neuronal cell cycle and proliferation studies.

For Research Use Only. Not for use in diagnostic procedures.

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