Thermo Fisher Scientific DDX5 Polyclonal Antibody
Rabbit polyclonal antibody against human DDX5 protein. Validated for WB, IHC (Paraffin), and IP applications. Recognizes residues 575–614 of DDX5. Supplied as liquid at 0.2 mg/mL, stored at 4°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 575 and the C-terminus (residue 614) of human DEAD (Asp-Glu-Ala-Asp) Polypeptide 5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan and Chimpanzee
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases involved in RNA secondary structure modification processes such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
Some members are implicated in embryogenesis, spermatogenesis, and cellular growth and division.
This gene encodes a DEAD box protein functioning as an RNA-dependent ATPase and proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.
The gene contains 13 exons, and alternatively spliced transcripts have been detected, though no isoforms are identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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