Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), eBioscience
Mouse CD90.2 (Thy-1.2) 단백질을 인식하는 30-H12 클론의 Rat IgG2b 단일클론 항체로, Flow Cytometry 및 IHC(F) 등 다양한 분석에 사용 가능. 0.5 mg/mL 농도의 액상 형태이며 PBS buffer에 sodium azide 포함. T 세포 및 신경세포 표지에 적합.
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Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 3 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 12 publications |
| Functional Assay | Assay-Dependent | 1 publication |
| Miscellaneous PubMed | - | 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-H12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467381 |
Available Formats
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The 30-H12 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (BALB/c, CBA, C3H, C57BL/6, C58/J, SJL, etc.). Strains expressing CD90.1 (PL, AKR) do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported:
Reported for use in flow cytometric analysis, immunohistochemical staining of frozen sections, and in vivo/in vitro depletion. For functional assays, use the Functional Grade purified 30-H12 (Product #16-0903).
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test and should be titrated for optimal performance.
Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily. Expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. Plays roles in cell-cell or cell-ligand interactions during synaptogenesis and other neural events. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic and neural cells, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cell lines. In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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