Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), FITC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 3 publications

Product Specifications

Additional Formats

• eFluor 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin).
GARP is an approximately 80 kDa glycoprotein expressed on the cell surface. Its expression has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression is observed on megakaryocytes, platelets, and activated regulatory T (Treg) cells.
GARP expression on activated Treg cells is necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells
• Recommended Use: 5 µL (0.5 µg) per test in a final volume of 100 µL
• Typical Cell Range: 10⁵–10⁸ cells/test
• Excitation: 488 nm
• Emission: 520 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

GARP (leucine rich repeat containing 32) is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region.
It was identified through gene expression profiling of Treg and Th cells following TCR stimulation.
Since GARP is expressed in CD4⁺, CD25⁺, FoxP3⁺ cells (Tregs), it serves as a potential cell surface marker for Treg cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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