Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.

• In B cells, IRF4 is highly expressed in mature plasma cells and regulates differentiation and expression of genes such as Prdm1 (Blimp1).
• In T cells, IRF4 contributes to Treg, Th2, Th9, and Th17 cell development, inducing Blimp1 and IL-10 expression.
• IRF4 also plays roles in macrophage polarization and is upregulated in various hematologic malignancies.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:

• Tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
• Recommended usage ≤0.5 µg per test (100 µL final volume).
• Optimal antibody concentration should be titrated per assay.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family of transcription factors contains a conserved tryptophan pentad repeat DNA-binding domain.

• Regulates interferon response and interferon-inducible gene expression.
• IRF4 is lymphocyte-specific and modulates TLR signaling, essential for innate and adaptive immune activation.
• Chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH loci is associated with multiple myeloma.
• Alternative splicing variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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