Thermo Fisher Scientific RAP80 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

2.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 17 amino acid peptide from near the amino terminus of human RAP80. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAP80,uniProtId:Q96RL1-1,ncbiNodeId:9606,antigenRange:1,antigenLength:719,antigenImageFileName:PA5-20414_RAP80_Q96RL1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20414_RAP80_Q96RL1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11156645

Product Specific Information

A suggested positive control is 293 cell lysate.

PA5-20414 can be used with blocking peptide PEP-0531.

Target Information

RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.