Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.05 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue & Yellow Series
• Pacific Orange
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells, playing a crucial role in T cell development and activation. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated for flow cytometric analysis of mouse thymocytes and splenocytes at ≤0.5 µg/test (100 µL final volume).

Usage Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Shipments include CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining performance.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.

Technology:
NovaFluor conjugates are based on Phiton™ technology using novel nucleic acid dye structures for engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD4 is a co-receptor for MHC class II recognition and HIV binding, primarily expressed on T helper lymphocytes, but also found in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific brain and gut regions. CD4 facilitates early T-cell activation via association with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple splice variants have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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