Thermo Fisher Scientific H3K27ac Polyclonal Antibody
H3K27ac에 특이적인 Rabbit Polyclonal Antibody로 Western Blot, ICC/IF, ELISA 등에 사용 가능. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며, -20°C 이하에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ELISA | 1:100 |
| Peptide Array | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to human histone H3, acetylated at lysine 27 (H3K27ac) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608320 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several types of post-translational modifications such as methylation, acetylation, and phosphorylation, influencing diverse cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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