Thermo Fisher Scientific mGluR1 Recombinant Rabbit Monoclonal Antibody (JM11-61)
mGluR1 단백질을 인식하는 재조합 토끼 단일클론 항체로 Western blot 및 IHC(파라핀 절편)에 적합. 높은 특이성과 재현성을 제공하며, Protein A로 정제된 액상 형태. 인간, 마우스, 랫트 시료에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM11-61 |
| Immunogen | Recombinant protein within Human mGluR1 (aa 280–420) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809912 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select optimal candidates for production.
Advantages:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the larger rabbit immune repertoire
Target Information
Glutamate is the main excitatory neurotransmitter in the brain. It acts on both ligand-gated receptor channels (NMDA, AMPA, and kainate receptors) and metabotropic glutamate receptors (mGluRs) coupled to G-proteins.
Group-I receptors (mGluR1a, mGluR5) stimulate phospholipase C, increasing phosphoinositide turnover and calcium release.
Group-II and -III receptors (mGluR2, mGluR3) inhibit adenylyl cyclase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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