Thermo Fisher Scientific IL-33 Polyclonal Antibody
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인간 IL-33 단백질을 인식하는 염소 유래 polyclonal antibody로, WB, IHC, ICC, ELISA, Neutralization 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 낮은 내독소 수준과 높은 특이성을 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 0.2–0.8 µg/mL |
| Neutralization (Neu) | 0.75–3.0 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human IL-33 (Ser112–Thr270) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609883 |
Product Specific Information
- In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse IL-33 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).
Target Information
IL-33 (Interleukin-33) is a 270 amino acid protein belonging to the IL-1 family with an IL-1-like C-terminal domain.
It functions as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
- Binds to IL1RL1/ST2 and activates NF-κB and MAP kinases.
- Induces TH2 cytokines (IL-4, IL-5, IL-13) and causes mucosal inflammation.
- Highly expressed in high endothelial venules of tonsils, Peyer’s patches, and mesenteric lymph nodes.
- Often migrates higher than predicted molecular weight in SDS-PAGE.
- May co-localize with heterochromatin and act as a transcriptional repressor.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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