Thermo Fisher Scientific IL-33 Polyclonal Antibody
Mouse IL-33에 특이적인 Thermo Fisher Scientific의 Goat Polyclonal Antibody로, WB, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 인간 IL-33과의 교차 반응이 2% 미만. Lyophilized 형태로 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.4 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | - |
| Flow Cytometry (Flow) | 2.5 µg per million cells | - |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) | View 1 publication |
| Neutralization (Neu) | 10–50 ng/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli derived recombinant mouse IL-33 (Ser109–Ile266) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2606911 |
Product Specific Information
- In sandwich ELISAs, less than 2% cross-reactivity with recombinant human IL-33 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-33 (Interleukin-33) is a 270 amino acid protein belonging to the IL-1 family with an IL-1-like C-terminal domain. It functions both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to IL1RL1/ST2, activating MYD88, IRAK1, IRAK4, and TRAF6, which leads to NF-κB and MAP kinase activation and TH2 cytokine production (IL-4, IL-5, IL-13).
IL-33 is expressed in high endothelial venules of tonsils, Peyer’s patches, and mesenteric lymph nodes, and is nearly undetectable in placenta. Prolonged IL-33 treatment in mice can cause eosinophilia, splenomegaly, and mucosal organ pathology.
Despite its predicted molecular weight, IL-33 often migrates at a higher molecular weight in SDS-PAGE. It can co-localize with heterochromatin and act as a transcriptional repressor, emphasizing its dual role as cytokine and nuclear factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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